拉夫堡大学在研究强度（REF2014）方面是英格兰排名前十的大学，并且有资格提交给REF的拉夫堡学术人员中有66％的杰出工作被评为“世界领先”。或“国际一流”，而全国平均水平为43％。<br>在选择拉夫堡进行研究时，您将与该领域的领导者一起工作。您将受益于我们的全面支持和指导，包括量身定制的职业建议，以帮助您在研究和未来职业中取得成功。<br>项目详细信息：<br>该博士项目涉及用于检测生物学上重要阴离子的发光受体的合成。这些分子将被用于实时监测酶反应，并可视化活细胞中的生化过程。我们将探讨受体结构和几何
Peroxynitrite (ONOO−) is a powerful and short-lived oxidant formed in vivo, which can react with most biomolecules directly. Elevated intracellular levels of ONOO− can lead to significant damage to lipids, proteins and DNA and has been implicated in several diseases including Alzheimer's and Parkinson's disease. To fully understand the roles of ONOO− in cell biology and disease, improved methods for the selective detection and real-time analysis of ONOO− are needed. Existing molecular probes suffer from short-lived fluorescence read-outs, which can be difficult to distinguish from the background fluorescence of biomolecules, and insufficient selectivity between reactive species (e.g. NO, hydroxyl radicals), precluding accurate measurements of ONOO–. This project will develop a water-soluble, luminescent probe capable of rapid and sensitive detection of peroxynitrite in human serum, living cells and biological matrices. We will utilise the long luminescence lifetime of the probe to record time-resolved measurements of ONOO–, in different cell types, using a standard microplate reader. This will provide a better understanding of the roles of RONSin cell biology and the mechanisms of transport of RONS into cells. Crucially, this could facilitate the development of more effective therapies and devices, tailored to delivering specific levels of RONS to targeted sites within cells. The student will have the possibility to spend time working in the laboratories of collaborators (UK and Germany), to test the probes in biological and cellular assays.